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robosep tm-s  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc robosep tm-s
    Robosep Tm S, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/robosep+tm-s/pmc12164055-11-4-1?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    robosep tm-s - by Bioz Stars, 2026-08
    90/100 stars

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    Cellular stress assay (CSA) with OptiPrep-isolated ( n = 302) and <t>Robosep-isolated</t> PBMCs ( n = 216). ( A ) Oxygen consumption rate (OCR) and ( B ) extracellular acidification rate (ECAR). The horizontal lines show the mean ± SD. FCCP = carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; SD = standard division; RotAA = rotenone and antimycin A.
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    Cellular stress assay (CSA) with OptiPrep-isolated ( n = 302) and Robosep-isolated PBMCs ( n = 216). ( A ) Oxygen consumption rate (OCR) and ( B ) extracellular acidification rate (ECAR). The horizontal lines show the mean ± SD. FCCP = carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; SD = standard division; RotAA = rotenone and antimycin A.

    Journal: International Journal of Molecular Sciences

    Article Title: Cellular Stress Assay in Peripheral Blood Mononuclear Cells: Factors Influencing Its Results

    doi: 10.3390/ijms232113118

    Figure Lengend Snippet: Cellular stress assay (CSA) with OptiPrep-isolated ( n = 302) and Robosep-isolated PBMCs ( n = 216). ( A ) Oxygen consumption rate (OCR) and ( B ) extracellular acidification rate (ECAR). The horizontal lines show the mean ± SD. FCCP = carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; SD = standard division; RotAA = rotenone and antimycin A.

    Article Snippet: PBMC isolation by negative selection using magnet beads was carried out with the Robosep TM -S system and the EasySep TM direct Human PBMC Isolation Kit (StemCell TM , Vancouver, Canada) according to the manufacturer’s protocol.

    Techniques: Isolation

    Cellular stress assay (CSA) with OptiPrep-isolated PBMCs ( n = 293) and Robosep-isolated PBMCs ( n = 185). ( A ) Proton production rate (PPR) and ( B ) respiratory quotient (RQ). The horizontal lines show the mean ± SD. It shows that the variation in proton production and extracellular acidification is much higher in Opti-isolated cells. FCCP = carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; SD = standard division; RotAA = rotenone and antimycin A.

    Journal: International Journal of Molecular Sciences

    Article Title: Cellular Stress Assay in Peripheral Blood Mononuclear Cells: Factors Influencing Its Results

    doi: 10.3390/ijms232113118

    Figure Lengend Snippet: Cellular stress assay (CSA) with OptiPrep-isolated PBMCs ( n = 293) and Robosep-isolated PBMCs ( n = 185). ( A ) Proton production rate (PPR) and ( B ) respiratory quotient (RQ). The horizontal lines show the mean ± SD. It shows that the variation in proton production and extracellular acidification is much higher in Opti-isolated cells. FCCP = carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; SD = standard division; RotAA = rotenone and antimycin A.

    Article Snippet: PBMC isolation by negative selection using magnet beads was carried out with the Robosep TM -S system and the EasySep TM direct Human PBMC Isolation Kit (StemCell TM , Vancouver, Canada) according to the manufacturer’s protocol.

    Techniques: Isolation

    Boxplots of the basal proton production rate (PPR) ( A ) and the basal respiratory quotient (RQ) ( B ) of PBMCs isolated by OptiPrep ® ( n = 293) density centrifugation or Robosep TM -S negative selection ( n = 185). The red line in B indicates an RQ of 1.0, which is related to the complete oxidation of glucose by the cells. The outliers (black dots) in ( A , B ) are defined as data points for PPR and RQ, respectively, located outside the whiskers of the boxplots. The greater the number of outliers, the more inconsistent the PPR and RQ results.

    Journal: International Journal of Molecular Sciences

    Article Title: Cellular Stress Assay in Peripheral Blood Mononuclear Cells: Factors Influencing Its Results

    doi: 10.3390/ijms232113118

    Figure Lengend Snippet: Boxplots of the basal proton production rate (PPR) ( A ) and the basal respiratory quotient (RQ) ( B ) of PBMCs isolated by OptiPrep ® ( n = 293) density centrifugation or Robosep TM -S negative selection ( n = 185). The red line in B indicates an RQ of 1.0, which is related to the complete oxidation of glucose by the cells. The outliers (black dots) in ( A , B ) are defined as data points for PPR and RQ, respectively, located outside the whiskers of the boxplots. The greater the number of outliers, the more inconsistent the PPR and RQ results.

    Article Snippet: PBMC isolation by negative selection using magnet beads was carried out with the Robosep TM -S system and the EasySep TM direct Human PBMC Isolation Kit (StemCell TM , Vancouver, Canada) according to the manufacturer’s protocol.

    Techniques: Isolation, Centrifugation, Selection